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(covers information from several alternate timelines)
The Vulcan salute was a hand gesture used by Vulcans. It involved holding the palm of one hand outwards while placing the fingers in a "V" shaped by separating the middle and ring fingers, while keeping the others together, with the thumb extended.
When parties took their leave of each other, one party could use the phrase "Peace and long life" and still receive "Live long and prosper" as a reply. (TOS: "Is There in Truth No Beauty?", "The Savage Curtain"; TNG: "Sarek", "Unification I") Alternately, each party could simply state to the other "Live long and prosper, (name)." (TOS: "Amok Time"; Star Trek: Voyager various episodes)
In diplomatic situations, the greeting used was "I/We (depending on situation) come to serve," possibly prefaced by the statement of the initiators' name. The response was "Your service honors us." (TOS: "Journey to Babel"; TNG: "Data's Day", "Sarek", "Unification I")
In 2151, an ornament of Surak performing the Vulcan salute with both hands was displayed aboard the Vahklas, a Vulcan civilian transport ship. Unlike other Vulcan salutes, Surak's hands were not held upright. (ENT: "Fusion")
Several Humans greeted Vulcans with the hand gesture, including Captain Jonathan Archer in 2154 (ENT: "Kir'Shara") and Captain Jean-Luc Picard in 2368. (TNG: "Unification I") T'Pol taught Trip the salute when she took him to Vulcan to meet her mother, T'Les. (ENT: "Home") Zefram Cochrane tried and failed to perform it in 2063, during First Contact; he settled on a handshake instead. (Star Trek: First Contact) The saying was quite well-known among those in Starfleet – in 2375, trapped in the Delta Flyer under layers of rock, two minutes before the air would run out, Tuvok told Tom Paris, "In accepting the inevitable, one finds peace," to which Paris responded that, if this was another Vulcan axiom, he would stick to "Live long and prosper." Fortunately, Paris had barely finished the sentence when he was interrupted by the sound of USS Voyager's phaser drills breaking through the rock, and those on board the Delta Flyer were safely beamed back to the ship. (VOY: "Once Upon a Time")
As a practical joke, Tom Paris and Harry Kim once reprogrammed Tuvok's security console so that it said, "Live long and prosper," whenever he accessed the internal sensors. They also reprogrammed his replicator the same way. (VOY: "Revulsion")
In 2258, in the alternate reality brought about by Nero's incursion, Spock, after speaking with his younger counterpart, made the hand gesture but said, "Good luck," as he felt it would be "oddly self-serving" to say the usual phrase to himself. (Star Trek)
Background information Edit
The Vulcan salute was devised by Leonard Nimoy, based on a gesture made by various Jewish denominations, including Orthodox and Conservative. In TV Land's The 100 Greatest TV Quotes & Catchphrases, William Shatner described the salute as a benediction, comparing it to the Sign of the Cross. The gesture actually forms the Hebrew letter "Shin" and represents the honorific title "Shaddai", which means "Almighty (God)." The hand gesture is traditionally used by the Kohanim (Hebrew "priests"), Jews of priestly descent, during a blessing ceremony performed during the prayer service of certain Jewish holy days. The Jewish blessing is done with both hands, with arms extended upward at roughly a forty-five-degree angle, rather than one hand held upright as in the Vulcan salute. Nimoy learned the gesture, which takes practice to do, from visiting his grandfather's synagogue as a child. In the video William Shatner and Leonard Nimoy: The Twenty-Five Year Mission, Nimoy stated, "It took me years of diligent practice and self-denial to be able to do that." 
The Vulcan salute wasn't originally in the script of "Amok Time", which called for Spock to walk up to T'Pau followed by them simply exchanging brief greetings. Leonard Nimoy thought this might be a good chance to bring something unique to the Vulcan people. When he spoke to the episode's director, Nimoy suggested – citing examples of other gestures conveying greetings, such as handshakes, salutes, and bows – that perhaps Vulcans would greet each other with the Jewish gesture he remembered from his childhood, and the director agreed to try it. However, the actress playing T'Pau, Celia Lovsky, initially couldn't perform the salute, presenting a problem for the production personnel. They solved it by using a simple camera trick where her hands were below camera frame while she used one of her hands to get the other hand in the proper position. The salute was established from then on. (Leonard Nimoy: Star Trek Memories)
William Shatner was unable to do the Vulcan salute. When Kirk (as played by Shatner) performs the salute in Star Trek III: The Search for Spock, it appears that fishing line holds two of his fingers together. In the video William Shatner and Leonard Nimoy: The Twenty-Five Year Mission, Shatner joked that the reason he couldn't do it was because, in Leonard Nimoy's words, it took "years of diligent practice and self-denial." During a June 2009 appearance on The Tonight Show with Conan O'Brien, Shatner demonstrated his inability to perform the salute, to which O'Brien responded with a perfect salute of his own.
Influence and other usage Edit
Armin Shimerman devised a Ferengi gesture inspired by the Vulcan hand salute. "I know that the Vulcan hand sign is universally recognized," he commented. "I thought, 'Let's see if we can find something like that to do.'" (Star Trek: Deep Space Nine Companion (citation needed • edit))
The Filipino greeting "Mabuhay" can also be roughly translated as "live long and prosper" (it literally means "Live", but the expression is meant to convey a wish for someone to have a long and prosperous life in order to truly "live"). 
In Act 5, Scene 3, line 42, of Shakespeare's Romeo and Juliet, Romeo says to Balthasar, "Live, and be prosperous, and farwell good fellow." 
- The Jewish Origin of the Vulcan salute - a very complete page by Rabbi Yonassan Gershom, with photos and diagrams of how the salute forms the Hebrew letter Shin, the use of the Blessing Hands gesture on Jewish gravestones and jewelry, etc.
- Names of God in Judaism at Wikipedia shows a clear illustration of the Judaic origin of the Vulcan salute