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(covers information from several alternate timelines)
A vaccine was a chemical preparation that instructed a healthy immune system how to fight a pathogen. Some vaccines were made of live bacilli, which had to be shipped in a stasis field. (TNG: "True Q")
In 2004, a group of Xindi-Reptilians who were gathering individuals of various blood types to create a bio-weapon explained away their activities to Loomis by claiming they were working on an experimental vaccine for which they did not have approval. (ENT: "Carpenter Street")
In 2152, after Crewman Fisher contracted Rigelian fever, Doctor Phlox administered a vaccine for the disease to the entire crew of Enterprise NX-01, in order to prevent an outbreak. Around a month later, this ended up saving the life of Ensign Travis Mayweather. He was abducted by an automated repair station repairing Enterprise, which intended to use his synaptic pathways to enhance its computer core processing power, and replaced with a facsimile designed to mimic a death from isolytic shock. Phlox realized the replica was not Mayweather when he noticed that microbes from the vaccine, which should have thrived on isolytic energy, were dead. Jonathan Archer and T'Pol managed to rescue Mayweather from the station's computer core before the brain damage became irreversible. (ENT: "Dead Stop")
In the alternate reality, McCoy gave Kirk a vaccine for a viral infection spread by Melvaran mud fleas, to which Kirk had a bad reaction resulting in swollen hands and numb tongue. Those symptoms hindered him when he attempted to warn Uhura about a possible Romulan attack, when Nero attacked Rura Penthe in 2258. (Star Trek)
In 2266, on a planet that looked exactly like Earth, Dr. McCoy successfully developed a vaccine that cured at least the fatal form of a particular virus. The virus became fatal when the infected individual entered puberty, and had been created as a result of a disastrous life prolongation project that had been conducted by scientists on the planet, sometime around 1960. (TOS: "Miri")
In 2364, Ligon II had a vaccine to cure Anchilles fever, which the population of Federation planet Styris IV was suffering from. The USS Enterprise-D was sent to Ligon II to negotiate with Lutan, leader of the Ligonians, to acquire the vaccine. (TNG: "Code of Honor")