In that year, this ship, along with the USS Renegade and the USS Thomas Paine, secretly rendezvoused with the USS Enterprise-D at Dytallix B. On the planet's surface, the four ship captains had a covert meeting in which they discussed the possibility of an alien conspiracy within Starfleet. Soon afterwards, the Horatio was destroyed with all hands in Sector 63 when the ship imploded. The destruction of the ship was recorded as a disturbance by the Enterprise's sensors. The Enterprise raced to the origin of the disturbance, and discovered the wreckage of the Horatio. The cause for the implosion was believed to have been due to sabotage at the hands of Horatio's first officer, whom Keel had suspected of being under the influence of an alien parasite. (TNG: "Conspiracy")
According to the Star Trek Encyclopedia, the Horatio had a registry number of NCC-10532. Despite not physically appearing on screen as an intact starship, the Horatio marked the first canon reference to the Ambassador-class in Star Trek.