The Third Shift Antimatter Operations Unit was an unit within the Engineering Division of Federation starships. This unit, active in the third duty shift, was responsible for antimatter operations. A typical unit might have six engineers. There was a shift supervisor, a safety officer, two operations engineers, and two warp field technicians.

In 2367, a duty roster of this unit's staff was on display in the ready room of the USS Enterprise-D. All were on-duty.

The staff members in 2367 were: