Mrs. Templeton was a character in Captain Janeway's Gothic holonovel. Mrs. Templeton tended Lord Burleigh's mansion and guarded the secrets of the house especially that of the late Mrs. Burleigh. She made life difficult for Janeway's character. She deeply disapproved of Janeway, who played the governess in the novel. (VOY: "Cathexis")

In 2372, Captain Janeway hallucinated that Mrs. Templeton appeared outside the holodeck and was trying to kill her. She told Janeway that she had come between Lord Burleigh and her. She claimed that she was supposed to be the one to take care of him. She accused Janeway of causing trouble and that the family didn't want any more trouble. (VOY: "Persistence of Vision")

Mrs. Templeton was played by returning Trek actress Carolyn Seymour.