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A subspace layer is a region of subspace.
In the same year, the "telepathic pitcher plant" entity appearing as a wormhole, sensed that Seven of Nine was immune to its manipulation. It convinced the crew of the Voyager, that Starfleet on the other side had asked to put her in stasis, because the wormhole passed through a layer of subspace monitored by the Borg and her neural transceiver might attract their attention. (VOY: "Bliss")