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A subroutine was a subprogram in a computer, often called up to execute by a higher level program. It consisted of instructions that performed a specific set of tasks for a main routine. Subroutines often accounted for the sentient qualities of AI.

In 2154, the warp matrix of the Enterprise NX-01 was compromised by a Klingon subroutine. It infiltrated the ship's command protocols to do so. (ENT: "Affliction")

In late 2375, Noah Lessing of the USS Equinox designed a subroutine to mask the internal sensors of the USS Voyager, allowing the Equinox crew to steal a modified field generator that provided protection against nucleogenic lifeforms. (VOY: "Equinox")

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