Starbase 2 was a Federation starbase administered by Starfleet. It had a well-equipped and -staffed medical facility. It was located seventy-two hours away from Camus II at warp 2.

In 2269, Commander Spock suggested taking Doctor Janice Lester (with James T. Kirk in her body, a fact unknown to anyone but the two of them at the time) to Starbase 2 instead of Benecia Colony, whose facilities were inferior. Given the nature of Lester's condition, "Kirk" (Lester in his body) was inclined to disagree, saying that "Lester" would die before they arrived. Spock suggested they travel at maximum speed, but "Kirk" dismissed his suggestions out of hand (wanting Kirk to die). (TOS: "Turnabout Intruder")

According to the reference book Star Trek: Star Charts (United Federation of Planets III), Starbase 2 was located in the Alpha Quadrant.
The short story in the comic "Focus on... Star Trek" (an introduction to the Star Trek: Year Four series) picks up where "Turnabout Intruder" left off. Starbase 2 is shown to be the location where Janice Lester and Doctor Arthur Coleman were placed into custody. It is depicted as a Deep Space K-7-type station.

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