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In 2365, when Lieutenant commanders Data and Geordi La Forge confronted a hologram of James Moriarty, he described himself as being like a spider who feels the strings vibrate when anyone new chances into his web. (TNG: "Elementary, Dear Data")
When Sito Jaxa suggested that it would be good to be a spider under the table of the observation lounge, Sam Lavelle compared this to be a fly on the wall. They were talking about the meeting of the senior staff in the observation lounge and were curious what they were talking about. (TNG: "Lower Decks")
A species of large spider was the primary food source on a planet in a subspace pocket, accessible by a subspace sinkhole. Noss, one of many trapped on the planet, used an electronic device to attract the spiders so she could spear them. She taught the technique to Tuvok and Tom Paris when they and The Doctor also crashed on the planet in 2375. (VOY: "Gravity")