Sector 21527 was a region of space. This sector contained an unspecified asteroid field. This sector was located in the region between Sectors 21166 and 22079.

In 2370, the USS Enterprise-D performed tests of new weapons systems in this sector. Later, when the ship's crew was disabled by Barclay's Protomorphosis Syndrome, the Enterprise-D became adrift in this sector. (TNG: "Genesis")

For the pre-mastered "Chain of Command, Part II", a star chart was displayed on the bridge of the Enterprise which supposedly showed the location of the McAllister C-5 Nebula. For the remastered version, the map was changed. The most notable change was the sector number, which was changed from Sector 21527 to Sector 71527.

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