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A red 2 message was an important transmission sent out by Starfleet Command to their vessels.
In 2267, after the universe had been momentarily "winked out" of existence through the actions of Lazarus, Starfleet Command sent the USS Enterprise a red 2 message along with a Code One Alert from Commodore Barstow, which Captain James T. Kirk had Lieutenant Uhura verify. The message signaled to Kirk that an invasion was imminent. (TOS: "The Alternative Factor")