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During an encounter in 2154 with the Orions, the Enterprise NX-01 found itself on the receiving end of a towing cable that was deployed from Harrad-Sar's barge. The Enterprise was able to break free after it sent a positron burst through its navigational deflector and along the towing cable, which resulted in the triggering of an energy cascade that successfully disabled the Orion's power systems. (ENT: "Bound")
The Delta Rana warship used jacketed beams of antiprotons and positrons as its main weapon and fired beams with the equivalent firepower of forty megawatts and later four hundred gigawatts at the USS Enterprise-D in 2366. (TNG: "The Survivors")
Positrons could be detected using a positron emission sensor. Doctor Beverly Crusher used this device in 2367 while undertaking an autopsy of the crew of the USS Brattain. The sensor was used for brain tissue cross-sections. (TNG: "Night Terrors")
While exploring a class T cluster in 2376, an away team on the Delta Flyer found displaced positrons on a piece of hull that had been sheared off the ship. One possible cause was an impact from dark matter, though the results were inconclusive. (VOY: "Good Shepherd")