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The midbrain was a portion of the central nervous system associated with vision, hearing, motor control, sleep/wake, alertness, and temperature regulation.

In the 22nd century, Vulcan mind melds were frowned upon partly because they caused a disruption of neuroelectric impulses in the Vulcan midbrain, which could lead to the early stages of Pa'nar Syndrome. (ENT: "Stigma")

In 2371, Dr. Julian Bashir implanted Bareil Antos with a positronic matrix which interfaced with his midbrain. (DS9: "Life Support")

Soon after, Ptera was placed in a Vhnori cenotaph to end her life as she was dying of a tumorous lesion to her midbrain. (VOY: "Emanations")

In 2373, in an imaginary scenario in which the Sacajawea had crashed causing the death of Kathryn Janeway, Kes informed The Doctor there was minimal electrical activity in Janeway's midbrain. (VOY: "Coda")

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