The Great Lakes was a group of five lakes that existed on Earth's North American continent along the border between the old nations of Canada and the United States of America. They were connected through various rivers that ultimately emptied into the Atlantic Ocean. (DS9: "Little Green Men")
On Earth Two, which was an exact replica of Earth, a series of lakes identical to the Great Lakes could be seen on that planet's northern continent. (TOS: "Miri")
The names of the five lakes shown are Ontario, Erie, Michigan, Huron, and Superior.
The Great Lakes are not mentioned in dialogue but can clearly be seen on a United States wall map in "Little Green Men".
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