Commander Donatra was an officer in the Romulan military, and commander of the IRW Valdore, a Romulan Warbird. In that capacity, she was a co-conspirator in the plot to elevate Shinzon to the position of praetor of the Romulan Empire in 2379, and, along with fellow-commander Suran, acted as a military advisor to Shinzon.
When Shinzon expressed skepticism regarding Suran's loyalty, he instructed Donatra to be vigilant regarding Suran's actions, and to eliminate him at the first indication of treachery. Donatra attempted to express a sexual interest in Shinzon, but was soundly rebuffed by him due to his loathing of all Romulans. This, along with her discovery that Shinzon was planning to use thalaron radiation to destroy all life on Earth, caused her own loyalty to waver. Later, she held a covert conversation with Suran, questioning whether they truly desired to stand by as Shinzon committed genocide.
Later, along with another Warbird, Donatra commanded the Valdore in assisting the USS Enterprise in combat against Shinzon's vessel, the Reman Warbird Scimitar, explaining to Captain Picard that the Empire considered Shinzon's coup a matter of "internal security." The Valdore was critically damaged, however, when Shinzon executed a maneuver to lure Donatra into close range of the Scimitar, then firing at point-blank range. This, along with the destruction of the other Warbird, left the Enterprise crew to face the Scimitar alone, although they were eventually successful in destroying its thalaron generator, causing the ship to explode. Afterward, Donatra dispatched shuttles with medical personnel and supplies to assist the Enterprise crew in recovering from the battle, and informed Captain Picard that he had earned "a friend" in the Romulan Empire. (Star Trek Nemesis)
- In the novelization of the film, Donatra's expanded back story is that her father, a prominent Romulan military commander, was "disappeared" (i.e., abducted and executed in secret) for daring to suggest a less oppressive response to a planetary rebellion to the praetor, when Donatra was only a young girl. Her mother placed her under the protection and guidance of Suran, who helped advance her career until she achieved the rank of commander at a surprisingly early age.
- In novels such as Star Trek: Destiny, Donatra leads the breakaway Imperial Romulan State in opposition to Tal'aura.
- In Star Trek Online, the Romulans reunify in 2385 following Tal'aura's assassination, with Donatra in control of the military and abolishing Reman slavery. She is missing and presumed dead following the supernova of 2387, but she turns up in the "Khitomer Accord" Special Task Force mission as a Borg drone. She commands an assimilated Scimitar-class ship.
- The novel Rough Beasts of Empire has Donatra arrested during the reunification of the Romulan people under Tal'aura in 2382, falsely accused of being behind an attempt to assassinate Spock, and committing suicide in her cell.