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A defense perimeter was an assortment of military units before or around a specific location, designated to defend this location against attacks. This could take place in space or on a planetary surface.
By 2374, Starbase 257 maintained a defense perimeter, which leaving or incoming vessels needed clearance to pass through. Vessels were supposed to stay at half impulse within the perimeter. (DS9: "Valiant")
In 2375, Captain Benjamin Sisko told Lieutenant Larkin, she had set up a solid defense perimeter around the communication array on AR-558, but needed to tighten up a flank. (DS9: "The Siege of AR-558")
In late 2375, the Dominion established a defense perimeter around Cardassia Prime. In orbit, Damar's attack ship was destroyed by Breen warships, part of the defense perimeter. After the Dominion's final retreat towards Cardassia Prime, the defense perimeter was composed of thousands of Jem'Hadar and Breen ships as well as orbital weapon platforms. (DS9: "The Dogs of War", "What You Leave Behind")