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Blinking was the regular closing and opening of one's eyes. To have something happen "in the blink of an eye" was for it to occur quickly. (DS9: "In the Cards" et al.) To not blink at taking an action was to do it without reserve. (DS9: "When It Rains...")
Data's blink rate was regulated by the Fourier series. In 2370, he noticed that his "mother"'s blinks had the same pattern, which clued him in to the fact that she was really an android that had replaced the Human woman. (TNG: "Inheritance")
Not blinking could refer metaphorically to a refusal to be the first to give in. In 2375, Admiral William Ross told Kira Nerys she could blink after Senator Kimara Cretak gave in to the demand to remove plasma torpedoes from Derna. (DS9: "Shadows and Symbols")
In 2376, while Neelix played a shell game with tera nuts in an attempt to confuse The Doctor, Tom Paris warned the hologram not to blink. The Doctor assured Paris that his optical subroutines were up to the task. (VOY: "Live Fast and Prosper")