Autosequencers were components of transporters and inertial dampers, responsible for computer-controlling the complex series of operations necessary for the correct operation of these devices.

In 2151, instituting a plan to rescue Captain Archer from a Suliban helix, Charles Tucker III brought the autosequencers of Enterprise NX-01's transporter online, preparing to beam Archer aboard. Although annular confinement was off by two microns, it was sufficient to rescue Archer, moments before he was about to be shot by Silik. (ENT: "Broken Bow")

In 2367, Enterprise tactical officer, Lieutenant Worf, boosted power to the transporter's autosequencers when it appeared that Ambassador T'Pel's signal was becoming lost while beaming aboard a Romulan Warbird. Despite Worf's actions, along with Lieutenant Commander Data engaging computer override, T'Pel's signal was apparently lost. A subsequent investigation indicated that the autosequencers were functioning normally. It was later learned, however, that T'Pel was, in fact, a Romulan spy, who had materialized safely aboard the Warbird. (TNG: "Data's Day")

In 2375, the autosequencers of the inertial dampers belonging to a shuttlecraft being piloted by Worf and Captain Picard was damaged by phaser fire from a scoutship piloted by Data. As both ships were locked together by docking clamps, and the inertial coupling was exceeding tolerance due to Data's evasive maneuvers, this necessitated Worf transferring the controls to manual in order to realign the autosequencers. Worf was subsequently able to reroute power to the dampers and stabilize both vessels before they crashed. (Star Trek: Insurrection)