(covers information from several alternate timelines)
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Assassination was the act of murdering an important individual for political or ideological purposes. Those who carried out assassinations were known as assassins, who may have been in the employ of a government or other group, or operated independently for hire.
Assassination was a common practice utilized in the mirror universe to secure promotions or rid oneself of those who might wish to do the same to you. Sylvia Tilly became captain of the ISS Discovery through assassinating the previous captain, who had been recovering from the Crestian flu.(DIS: "Despite Yourself") In 2267, when Captain James T. Kirk, accidentally transported to that universe, refused to fire on the Halkans for denying the Terran Empire dilithium, Ensign Pavel Chekov attempted to assassinate him. He stated that nobody would question the assassination of a captain who had disobeyed prime orders of the Empire. Later, on researching the history of that universe, Kirk discovered that his counterpart had advanced to the command of the ISS Enterprise by assassinating Captain Christopher Pike. (TOS: "Mirror, Mirror")
In 2365, a conference on Pacifica was almost threatened when two Antideans posed as visiting dignitaries, intending to blow up the area with ultritium hidden in their robes. They were identified by Ambassador Lwaxana Troi and detained. (TNG: "Manhunt")
By 2366, Dukat had survived four assassination attempts during his time as Prefect of Bajor. Timor Landi, Ishan Chaye and Jillur Gueta were executed for an attempt in that year. (DS9: "Things Past", "Waltz")
The same year, a Bajoran officer tried to assassinate Commander Benjamin Sisko with a small device he received from Doctor Julian Bashir. All participants were under the influence of the Saltah'na energy spheres. (DS9: "Dramatis Personae")
In 2371, Enabran Tain ordered the assassinations of several former associates, including Garak, who posed potential threats to his return to power. Garak was able to preempt his assassination attempt by the Flaxian Retaya by destroying his own shop. (DS9: "Improbable Cause", "The Die is Cast")
In 2374, the Orion Syndicate attempted to assassinate the Klingon ambassador to Farius Prime at the behest of the Dominion. The plan failed due to the actions of Miles O'Brien. (DS9: "Honor Among Thieves")
In late 2375, Luther Sloan and Section 31 duped Julian Bashir into believing they were about to assassinate Tal Shiar Chairman Koval. In reality, they hoped to stop Kimara Cretak from taking Koval's seat on the Continuing Committee. (DS9: "Inter Arma Enim Silent Leges")
In 2379, after Praetor Hiren rejected their demands by Commander Suran to forge an alliance with the Remans against the Federation, Senator Tal'aura used a thalaron radiation projector to assassinate the entire Romulan Senate. (Star Trek Nemesis)