American Sign Language was a type of sign language used in the United States, Canada, and various non-English-speaking countries on Earth.

The educational background of Kathryn Janeway included study in American Sign Language, chromolinguistics, and the gestural idioms of the Leyron. Despite this experience, she could not get the hang of the Tak Tak's form of communication in 2373. (VOY: "Macrocosm")

While playing Riva, actor Howie Seago used ASL. However, it is unsure if this was in fact the unknown language Riva was using, because when Data was tasked to find out what language it was, one language he examined was M-9, most gestures which were seen on-screen were those used in ASL. (Star Trek Encyclopedia (4th ed., vol. 2, p. 6))

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